I work in a biochemistry lab and we often have to fit equations to data and determine the goodness of fit. In this set of slides I show a plotly plot that allows the user to zoom in on fitted data to determine how good the fit is.
12/26/2018
I work in a biochemistry lab and we often have to fit equations to data and determine the goodness of fit. In this set of slides I show a plotly plot that allows the user to zoom in on fitted data to determine how good the fit is.
This equation was used to fit the data on the next slide:
\(Fraction\ Bound = \frac{X}{K_a + X}\), whereby \(X\) is the concentration of a binding partner (the glucocorticoid receptor in this plot) and \(K_a\) is the affinity constant–an empirical value that describes how strongly two proteins interact.
The fitted \(K_a\) is 15.6 \(Molar^{-1}\).